Biochemistry and Molecular Biology
Location: Boston, MA, United States
School: Boston University
Field of study: Biochemistry and Molecular Biology


Boston University, Boston, MA September 2014-May 2017
Candidate of B.Sc. Biochemistry & Molecular Biology
University of New England, Biddeford, ME August 2014-May 2014
Pre-pharmacy cGPA 3.81
Relevant Coursework

• Qualitative Analytical Chemistry
• Organic Chemistry
• Biochemistry
• Physical Biochemistry
• Intensive Genetics
• Intensive Cell Biology
• Molecular Biology
• Microbiology
• Ecology and evolution
• Developmental Biology
• Immunology
• Statistics in life sciences
• Calculus
• Physics
• Medical ethics
• Principles of nutrition
• Health and disability across the lifespan
• Sociology, Public Speaking and Psychology

Lab Skill

• Cell culture
• Subcloning
• Transformation
• Transfection
• Mini-prep and maxi-prep isolation of plasmids
• Ligation and restriction enzyme digest
• DNA sequencing
• Protein purification and modification
• Nelson-Somogyi
• Glucose Oxidase
• Protein & chemical crystallization
• Gel electrophoresis
• Centrifugation
• Nanometer and Nano drop
• Affinity chromatography
• Activity assay
• Coupled assay
• In Vitro Transcription and Translation
• Wheat germ lysate-translation of proteins
• Bradford assay
• Lowry assay
• Zymography (native zone electrophoresis)
• Anaerobic chamber cell culture
• Autoclaving and aseptic technique
• Titration
• Layering & proteolysis
• Autoradiography
• Liquid scintillation counting
• TCA precipitation
• Thin Layer Chromatography (TLC)
• Western blot
• Acid-Schiff (PAS) staining
• Kirby-Bauer
• ImViC
• Polymerase chain reaction (PCR)

Experience in Biochemistry, Microbiology and Molecular Biology
• Designed and performed colorimetric and spectrophotometric protein assays, including preparation of standard curves and application of Beer’s law in order to compare colorimetric and spectrophotometric protein assays for linearity, sensitivity, and specificity.
• Determined apparent molar extinction coefficient of NADH
• Operated and calibrated glass-electrode pH meters to measure pH values of dilute aqueous solutions accurately and observed the selectivity of the glass electrode in extreme pH solutions and to un-buffered liquids.
• Prepared crude extracts of bovine heart muscle for the purification of enzyme lactate dehydrogenase (LDH) with ammonium sulfate fractionation and dialysis of LDH fractions for affinity chromatography to calculate enzyme activity and assay the effectiveness of procedural technique
• Performed unknown inhibitor assays to characterize their action on enzymes
• Performed gel exclusion chromatography using a Sephadex G-150 matrix column to assay eluted protein fractions to determine apparent molecular weight of a protein in native state
• Prepared, poured, and ran 12% and 15% SDS polyacrylamide gels to visualize proteins using Coomassie staining to determine protein purity and subunits
• Separated isozymes of protein using cellulose-acetate electrophoresis and visualized isozymes via activity stain specific for that protein
• Isolation of plasmid DNA (pcDNA-FLAG, pGEM3, pGEM4) using mini-prep, maxi-prep (ZymoPURE) and PCR to determine the quality of plasmid preparation and yield by measuring the plasmid DNA concentration using UV absorbance using 0.8%, 1% and 1.2% agarose-gel electrophoresis stained with ethidium bromide
• Performed restriction endonuclease digestions to determine size of DNA fragments to confirm plasmid identity and successful transformation and transfection
• Created a restriction map for restrictions in plasmid pcDNA-FLAG using SnapGene
• Prepared blood samples via deproteinization of mammalian blood to determine sugar concentration using the Nelson-Somogyi and glucose oxidase methods.
• Performed a combined RNA polymerase transcription and wheat germ lysate translation system for synthesis of proteins from genes cloned into plasmid pGEM3 and pGEM4
• Measured amount of radioactive methionine incorporated into protein via scintillation counting and autoradiography
• Measured protein modification using IAM and investigated the nature of excess coupling enzymes
• Isolated erythrocytes and plasma membranes to qualitatively separate phospholipids via TLC and determine glycoproteins via periodic acid-Schiff (PAS) staining and western blot
• Cultured DH5α strain of E.coli using LB broth and performed gram stains, IMViC tests and Kirby-Bauer method to characterize gram-negative and gram-positive bacteria.
Psychology Research
Harvard University’s Laboratory for Developmental Studies Cambridge, MA
Research Assistant September 2015 – September 2016
• Conducted research studies on cognitive associations and disassociations between unergative and unaccusative verbs in the Cambridge community
• Maintained lab duties that included organization of rooms, payment and appointment scheduling.
• Communicated with participants via SONA, FILEMAKER database, and confirmation phone calls
• Used the Tobii eye tracking device for research studies and interviewed participants on their thoughts on the study for revision in future experiments.
Clinical Experience
Brigham and Women’s Hospital, Medical Career Exploration Program Boston, MA
Volunteer Transporter and Patient Ambassador December 2015 - Present
• Transport patients, blood, and equipment. Allows for direct patient contact.
• Direct patients, families, and staff to different departments of the hospital and other hospitals in the Longwood community
• Maintain a safe and clean environment
• Direct contact with physicians, nurses and researchers.
Heywood Hospital Gardner, MA
Surgical Day Care, Front Desk, and Emergency Room Volunteer Winter and Summer 2015
• Observed clinical rounds and was given direct explanations of each patient’s symptoms and diagnosis.
• Change stretchers, sanitize discharge and patient rooms
• Consult with nurses before allowing visitors into the emergency room and nurse’s station.
• Supervised children and families in the waiting room at the emergency department.
Computer Skill

• Microsoft Excel
• Word
• PowerPoint
• LabScribe
• Latex
• E Prime programming language
• GenBank database
• PDB database
• “O” molecular modeling
• Quanta/CHARMm
• SnapGene

Volunteer Experience in Environmental Sustainability
Quabbin Organic Gardening and Composting Program Barre, MA
Intern 2011-2013
• Processed medicinal herbs, grains, vegetables and teas via oil extraction and infusion to create medicinal salves, creams and tinctures
• Maintained sale/consumer reports.
• Invested 400+ hours of gardening in the community garden and promoted agricultural sustainability of communities in Worcester county
Listening and Wellness Center, Barre, MA
Volunteer June-July 2013
• Developed and constructed a composting program for clients at the yoga and meditation facility.
Extracurricular Activities
Crafts for Charity Boston, MA
Member 2015-present
• Crochet hats and toys for children of local hospitals and organizations.
Global Apps Initiative Boston, MA
Member September 2014-December 2016
• Assisted in the creation of mobile apps for local non-for profit organizations.
Garlic and Arts Festival Orange, MA
Volunteer 2011-2013
• Managed sale of natural products that included chamomile and mint tea, shallot braids, medicinal salves, herbs, and vegetables produced from the community garden and gave presentations on composting and sustainability.