Research Associate
WORK HISTORY IN ACADEMIA AND BIOTECH COMPANIES
9/98 - 8/02 Research Specialist II for Brian K. Kobilka, M.D., Nobel Prize, Chemistry 2012
Stanford University, Stanford, CA http://med.stanford.edu/kobilkalab/
Maintained colonies of adrenergic knockout mice (?1, ?2, ?1?2, ?2A, ?2B, ?2C, & ?2A?2C).
Distributed knockout mice with protocols for genotyping to the Jackson Laboratory ------------/ , interested collaborators and other investigators.
Replaced Southern blot genotyping with PCR genotyping for mice colonies.
Expressed ?2-adrenoceptor in Pichia pastoris.
Made TEV (Tobacco Etch Virus) mutants of the human ?2- adrenoceptor to study ligand receptor interactions.
Responsible for safe use and disposal of all chemicals and radioactive materials.
7/98 - 9/98 Temp employed through BioSource assigned at Affymetrix, Santa Clara, CA
Designed PCR primers for quality control (Q.C.) of RNA.
Prepared total RNA and did Q.C. by RT_PCR, electrophoresis, UV spectrophotometry & Northern blotting.
Genechip� analyzed mRNA in-house and for Stanford University.
4/98 - 6/98 Intern at Genetic Information Research Institute, Mountain View, CA
Created a database of bacterial repetitive elements.
Selected and scored new consensus sequences for human repetitive sequence database. Expanded repetitive sequences to create new consensus sequences.
7/92 - 5/95 Research Assistant II, CLONTECH Laboratories, Mountain View, CA
Began development of Tet-On/Tet-Off� gene expression system.
Began development of GenomeWalker� Universal Kit.
Co-developed The Delta RNA� Fingerprinting Kit.
Sequenced 2.6 kb ?-amylase gene, Sanger method.
Developed Human yeast artificial chromosome (YAC) DNA PCR screening kit.
5/92 - 6/92 Temp at SangStat Medical, Mountain View, CA
Development of cartridge based rapid detection ELISA.
01/92 - 5/92 Temp at ESCAgenetics, San Carlos, CA
Development of competitive Taxol ELISA. Developed HPLC validation assay for Taxol. Created standard operating procedures (SOP).
09/91 - 10/91 Temp employed through Manpower at Institute of Cancer and Developmental Biology, Syntex, Palo Alto, CA
Developed assays for evaluating monoclonal and polyclonal antibodies to screen tumors using: affinity chromatography, ELISA, SDS PAGE, Western blots, serum protein electrophoresis, & radial immunodiffusion.
PUBLICATIONS
Luda B. Diachenko, John Ledesma, Alex A. Chenchik, Paul D. Siebert,
Combining the technique of RNA fingerprinting and differential display to obtain differentially expressed mRNA. Biochemical and Biophysical Research Communications, Volume 219, Issue 3, 27 February 1996, Pages 824-828.
EDUCATION
MS Physics, Portland State University, Portland, Oregon, 2013.
BS Electrical Engineering, Portland State University, 2013.
BS Microbiology, Brigham Young University, Provo UT, 1989.
WORK HISTORY IN ACADEMIA AND BIOTECH COMPANIES
9/98 - 8/02 Research Specialist II for Brian K. Kobilka, M.D., Nobel Prize, Chemistry 2012
Stanford University, Stanford, CA http://med.stanford.edu/kobilkalab/
Maintained colonies of adrenergic knockout mice (?1, ?2, ?1?2, ?2A, ?2B, ?2C, & ?2A?2C).
Distributed knockout mice with protocols for genotyping to the Jackson Laboratory ------------/ , interested collaborators and other investigators.
Replaced Southern blot genotyping with PCR genotyping for mice colonies.
Expressed ?2-adrenoceptor in Pichia pastoris.
Made TEV (Tobacco Etch Virus) mutants of the human ?2- adrenoceptor to study ligand receptor interactions.
Responsible for safe use and disposal of all chemicals and radioactive materials.
7/98 - 9/98 Temp employed through BioSource assigned at Affymetrix, Santa Clara, CA
Designed PCR primers for quality control (Q.C.) of RNA.
Prepared total RNA and did Q.C. by RT_PCR, electrophoresis, UV spectrophotometry & Northern blotting.
Genechip� analyzed mRNA in-house and for Stanford University.
4/98 - 6/98 Intern at Genetic Information Research Institute, Mountain View, CA
Created a database of bacterial repetitive elements.
Selected and scored new consensus sequences for human repetitive sequence database. Expanded repetitive sequences to create new consensus sequences.
7/92 - 5/95 Research Assistant II, CLONTECH Laboratories, Mountain View, CA
Began development of Tet-On/Tet-Off� gene expression system.
Began development of GenomeWalker� Universal Kit.
Co-developed The Delta RNA� Fingerprinting Kit.
Sequenced 2.6 kb ?-amylase gene, Sanger method.
Developed Human yeast artificial chromosome (YAC) DNA PCR screening kit.
5/92 - 6/92 Temp at SangStat Medical, Mountain View, CA
Development of cartridge based rapid detection ELISA.
01/92 - 5/92 Temp at ESCAgenetics, San Carlos, CA
Development of competitive Taxol ELISA. Developed HPLC validation assay for Taxol. Created standard operating procedures (SOP).
09/91 - 10/91 Temp employed through Manpower at Institute of Cancer and Developmental Biology, Syntex, Palo Alto, CA
Developed assays for evaluating monoclonal and polyclonal antibodies to screen tumors using: affinity chromatography, ELISA, SDS PAGE, Western blots, serum protein electrophoresis, & radial immunodiffusion.
PUBLICATIONS
Luda B. Diachenko, John Ledesma, Alex A. Chenchik, Paul D. Siebert,
Combining the technique of RNA fingerprinting and differential display to obtain differentially expressed mRNA. Biochemical and Biophysical Research Communications, Volume 219, Issue 3, 27 February 1996, Pages 824-828.
EDUCATION
MS Physics, Portland State University, Portland, Oregon, 2013.
BS Electrical Engineering, Portland State University, 2013.
BS Microbiology, Brigham Young University, Provo UT, 1989.