Joseph Nicotra
Joseph Robert Nicotra
2609 SE 21st Place, Cape Coral, Florida, 33904
Phone: ------------
Email: ------------
Education:
University of North Carolina Wilmington (May 2015)
Bachelor of Science: Marine Biology
Bachelor of Science: Oceanography
Minor: Chemistry
GPA: 3.641
Research Experience:
Shellfish Hatchery Research at CREST in Wilmington, North Carolina (2015)
• Designed and performed study isolating various algae species as feed to optimize bay scallop growth.
• Performed hemocytometer cell counts to determine optimal daily feeding volume.
Work Experience:
Algenol Biotech, LLC in Fort Myers, Florida July 2015-Present
Operations Associate: Nov 2018-Present
• Plan and lead commissioning of upstream commercial GMP manufacturing plant.
• Operate and maintain three porphyridium photobioreactor systems.
• Operate ultrafiltration and centrifugation systems to extract exopolysaccharides from algal centrate.
• Formulate, sterilize, and package product according to customer and GMP standards.
• Manage company-wide change order program.
• Manage and perform weekly sampling and monitoring of recovery and injection wells in compliance with federal permits.
• Train new operators and interns.
• Conduct routine water quality monitoring and maintenance on water softening, water ultrafiltration, and reverse osmosis systems.
• Troubleshoot and execute basic repairs on system plumbing and equipment.
• Monitor culture health through daily culture sample analyses.
• Write and optimize SOPs and operational checklists for upstream and downstream processing.
• Create facility safety documentation including lockout tagout procedures and confined space evaluations and permits.
• Participate in value stream mapping (VSM) exercises to cut plant operational costs through material, design, and operational improvements.
• Lead root cause analyses (RCA).
• Maintain manufacturing plant supplies inventory program.
• Perform on-call response duties after hours on weekdays, weekends, and holidays.
Operations Assistant: Dec 2015-Nov 2018
• Assisted construction and deployment of experimental photobioreactor systems.
• Operated upstream commercial plant demonstration system for phycocyanin (algal food coloring pigment) production.
• Commissioned and operated R&D scale, semi-automatic, photobioreactor system, growing biomass for crude oil production.
• Performed small-scale experiments to optimize nutrient media and maximize biomass productivity.
• Composed and published comprehensive phycocyanin commercial demonstration report.
Operations Intern: July 2015-Dec 2015
• Assisted CIP and inoculation operations of two-acre algae grow-field for ethanol production.
• Performed repairs and routine maintenance on photobioreactors and supporting tank and filtration systems.
Bait Shop Attendant at Big Boys Bait & Tackle, Cape Coral, FL: Part-time, 2018-Present
• Receive and sort shrimp, crabs, pinfish, and shiners.
• Maintain bait holding tanks and associated filters/skimmers/pumps.
• Perform customer service and cashier duties.
• Tie and maintain inventory of custom flies.
Foreman at Florida Sealcoat and Striping: Summers, 2012-2015
• Cleaned, sealed and striped parking lots.
• Performed asphalt and concrete repair.
• Supervised small team to perform work to blueprint specifications.
Skills:
• Fieldwork, wet work, and working in harsh Florida environment.
• Good manufacturing practice (GMP) standards knowledge
• Operating in GMP facility and clean room environment.
• Clean in place (CIP) and aseptic techniques.
• Composing and executing equipment qualification plans (EQP).
• Basic PVC plumbing construction and repair.
• Interpreting P&IDs and process flow diagrams.
• Dead end filtration, filter integrity testing, and filter maintenance.
• Using an HMI to conduct automated or remote operations.
• NetFacilities CMMS software.
• 5S lean manufacturing methodology.
• Scientific writing and presenting scientific research.
• Microsoft Programs: Word, Excel, Powerpoint, Outlook
• Safety Training/Certifications: confined space, arc flash, HAZWOPER, lockout tagout, forklift, fall arrest, chemical spill response, CPR/first aid.
• Laboratory Analyses/Skills: optical density, salinity, pH, alkalinity, conductivity, viscosity, ORP, TSS, chlorine, turbidity, centrifugation, microscopy, cell counts, microbe plating, pipetting, serial dilutions, sterile filtration, autoclave operation, balance operation, lab equipment calibration.
Joseph Robert Nicotra
2609 SE 21st Place, Cape Coral, Florida, 33904
Phone: ------------
Email: ------------
Education:
University of North Carolina Wilmington (May 2015)
Bachelor of Science: Marine Biology
Bachelor of Science: Oceanography
Minor: Chemistry
GPA: 3.641
Research Experience:
Shellfish Hatchery Research at CREST in Wilmington, North Carolina (2015)
• Designed and performed study isolating various algae species as feed to optimize bay scallop growth.
• Performed hemocytometer cell counts to determine optimal daily feeding volume.
Work Experience:
Algenol Biotech, LLC in Fort Myers, Florida July 2015-Present
Operations Associate: Nov 2018-Present
• Plan and lead commissioning of upstream commercial GMP manufacturing plant.
• Operate and maintain three porphyridium photobioreactor systems.
• Operate ultrafiltration and centrifugation systems to extract exopolysaccharides from algal centrate.
• Formulate, sterilize, and package product according to customer and GMP standards.
• Manage company-wide change order program.
• Manage and perform weekly sampling and monitoring of recovery and injection wells in compliance with federal permits.
• Train new operators and interns.
• Conduct routine water quality monitoring and maintenance on water softening, water ultrafiltration, and reverse osmosis systems.
• Troubleshoot and execute basic repairs on system plumbing and equipment.
• Monitor culture health through daily culture sample analyses.
• Write and optimize SOPs and operational checklists for upstream and downstream processing.
• Create facility safety documentation including lockout tagout procedures and confined space evaluations and permits.
• Participate in value stream mapping (VSM) exercises to cut plant operational costs through material, design, and operational improvements.
• Lead root cause analyses (RCA).
• Maintain manufacturing plant supplies inventory program.
• Perform on-call response duties after hours on weekdays, weekends, and holidays.
Operations Assistant: Dec 2015-Nov 2018
• Assisted construction and deployment of experimental photobioreactor systems.
• Operated upstream commercial plant demonstration system for phycocyanin (algal food coloring pigment) production.
• Commissioned and operated R&D scale, semi-automatic, photobioreactor system, growing biomass for crude oil production.
• Performed small-scale experiments to optimize nutrient media and maximize biomass productivity.
• Composed and published comprehensive phycocyanin commercial demonstration report.
Operations Intern: July 2015-Dec 2015
• Assisted CIP and inoculation operations of two-acre algae grow-field for ethanol production.
• Performed repairs and routine maintenance on photobioreactors and supporting tank and filtration systems.
Bait Shop Attendant at Big Boys Bait & Tackle, Cape Coral, FL: Part-time, 2018-Present
• Receive and sort shrimp, crabs, pinfish, and shiners.
• Maintain bait holding tanks and associated filters/skimmers/pumps.
• Perform customer service and cashier duties.
• Tie and maintain inventory of custom flies.
Foreman at Florida Sealcoat and Striping: Summers, 2012-2015
• Cleaned, sealed and striped parking lots.
• Performed asphalt and concrete repair.
• Supervised small team to perform work to blueprint specifications.
Skills:
• Fieldwork, wet work, and working in harsh Florida environment.
• Good manufacturing practice (GMP) standards knowledge
• Operating in GMP facility and clean room environment.
• Clean in place (CIP) and aseptic techniques.
• Composing and executing equipment qualification plans (EQP).
• Basic PVC plumbing construction and repair.
• Interpreting P&IDs and process flow diagrams.
• Dead end filtration, filter integrity testing, and filter maintenance.
• Using an HMI to conduct automated or remote operations.
• NetFacilities CMMS software.
• 5S lean manufacturing methodology.
• Scientific writing and presenting scientific research.
• Microsoft Programs: Word, Excel, Powerpoint, Outlook
• Safety Training/Certifications: confined space, arc flash, HAZWOPER, lockout tagout, forklift, fall arrest, chemical spill response, CPR/first aid.
• Laboratory Analyses/Skills: optical density, salinity, pH, alkalinity, conductivity, viscosity, ORP, TSS, chlorine, turbidity, centrifugation, microscopy, cell counts, microbe plating, pipetting, serial dilutions, sterile filtration, autoclave operation, balance operation, lab equipment calibration.