Research associate, scientist, PhD
||New York, NY, United States
||Catholic University of Sacred Heart (Rome, Italy)
||Biotechnology, Molecular Pathology
Research associate, scientist, PhD
SUMMARY OF QUALIFICATIONS
Organized, team-oriented and thorough scientist whose research activity has been focused on cancer biology study. A nine year career as an experienced molecular biologist, most recently as a research associate at Danbury Hospital, a branch of the Western Connecticut Health Network. During the five years at Danbury Hospital, had eleven research papers published in peer-reviewed journals. Studies focused on the mechanisms through which drug resistance occurs in cancer patients, particularly the role of protein over-expression in drug resistant tumors. Final aim of the studies were the investigation of protein interactions in drug resistant ovarian and colon cancer, as well as other solid malignancies, to develop a protein interaction inhibitor to reverse the drug resistance. Latest experimental project utilized patients and cellular models to develop a set of disease-related bio-markers of drug resistance, combining micro-RNA and gene expression analysis.
Highly driven and flexible professional willing to take on diverse challenges, work independently, be responsible for developing projects, and meeting deadlines.
• Fluigdim tecnology experience: nanofluidic gene and micro-RNA expression (Biomark system), Single-Cell miRNA Profiling and Targeted Gene Expression (C1™ System Fluidigm), Digital PCR (qdPCR 37K™ IFC array)
• Protein purification manual and automatic system Profinia, , gel electrophoresis, SDS-PAGE, ELISA, Western blotting, Far Western blotting and coimmunoprecipitation of proteins. Protein interaction study with Proteon SPR (BioRAD).
• Library preparation for Illumina sequencing, recombinant DNA techniques and exosome purification from serum/blood/urine with recovery of DNA and micro-RNA.
• Strong interpersonal communication skills resulting in effective audience appropriate presentations and successful collaborations with scientific and administrative supervisors and colleagues.
Western Connecticut Health Network, Danbury, CT February 2011 – August 2016.
Research Associate: Research in cancer biology using molecular biology techniques, as well as cellular biology, biochemistry, and chemistry. Led the project development and experimental set up of the following studies: protein-protein interaction in solid malignancies, screening of potential inhibitors, and development of disease-related bio-markers based on gene and microRNA expression. Emphasis placed on protein purification, SPR tecnology, library preparation, Fluidigm Tecnology (Biomark system) and C1 Fluidigm for single cell analysis.
Zhermack Spa, Rovigo (Italy) September 2008 - October 2009.
Chemical Analyst: Operated in a Quality Control laboratory, responsible for analyzing alcohol based solvents, sanitary liquid detergent/disinfectant, and silicone based resins for dentistry implantology. Lab activities included HPLC analysis, Gas-Chromatography analysis, InfraRed analysis, Atomic Absorption to determine that the raw material and final product met distributor’s standard of quality and the purity required.
University of Padua (Padua, Italy) February 2008 – September 2008.
Research Fellow: Employed in Department of Public Health, Comparative Pathology and Veterinary Hygiene. Research activity in food safety focused on the development of molecular biology assay for allergenic molecules in food and assessment of hygienic risk during the food processing (HACCP). Molecular biology activity: PCR, Real Time PCR, Kit ELISA, Western Blotting, bioinformatics tools.
University of Padua (Padua, Italy) December 2007 – January 2008.
Volunteer: Operated as Lab Technician in the Department of Public Health, Comparative Pathology and Veterinary Hygiene. Development of microbiological and molecular biology based assay for the assessment of Hazard Analysis & Critical Control Points (HACCP): analysis and control of biological, chemical, and physical hazards from raw material to product in food production industry.
Catholic University of Sacred Heart (Rome, Italy) October 2010-December 2013
PhD in Molecular pathology in oncologic gynaecology. Final test on March 10th 2014. PhD thesis: Aza-Podophyllotoxins and drug resistance in ovarian cancer. (thesis supervisor Prof. C. Ferlini).
University of Padua (Padua, Italy) September 2005-October 2007
Master’s Degree in Biotechnologies for safety and food hygiene. Final grade 110 / 110 cum Laude. Experimental thesis: Search for a partner of cellular protein NS1-A of Avian Influenza Virus for a study of species-specific pathogenic. (thesis supervisor Prof. A. Calistri).
University of Padua (Padua, Italy) September 2002-September 2005
Bachelor’s Degree in Health Biotechnologies. Final grade 105 / 110. Experimental thesis: Construction of two BUD 32 gene mutant strains of Saccharomyces Cerevisiae. (thesis supervisor Prof. G. Sartori).
Technical Institute I.T.I.S F. Viola (Rovigo, Italy) July 2002
Certified Chemist Technician (Perito chimico industriale). Final grade 92 / 100.
Skills and competences
Molecular biology: Extraction and purification of nucleic acid, molecular biology and recombinant DNA techniques, DNA restriction analysis, DNA sequencing, PCR, RT-PCR, qPCR, RNA and DNA extraction from FFPE and serum/plasma, DNA quantification using Agilent 2100 BioAnalyzer, nanofluidic gene and micro-RNA expression using the Biomark system (Fluidigm), SNP analysis, Access Array for the preparation of samples for sequencing, Library preparation for Illumina sequencing, Single-Cell miRNA Profiling, Single-Cell Targeted Gene Expression and Single-Cell mRNA Sequencing using the C1™ System (Fluidigm), Digital PCR with new qdPCR 37K™ IFC array, gel electrophoresis, SDS-PAGE, Western blotting, Far western blotting, coimmunoprecipitation of proteins, DNA methylation and exosome purification from serum/blood/urine with recovery of DNA and micro-RNA. Protein purification with manual and automatic system Profinia (BioRad), use of Proteomic Platform instrument (MicroStar Lab, Hamilton) and proteomic analysis instrument (ProteOn, BIORAD).
Cell Biology: Culture of various mammalian cell lines, drug treatment and harvesting of cells, clonogenic assay.
Microbiology: Microbiological manipulation in a sterile environment, transformation of competent cell with plasmid DNA, microbiology techniques. Cell culture for bacterial and eukaryotic cell, cell transfection.
Chemistry: HPLC analysis (isocratic and gradient elution for Normal phase HPLC and Reverse phase HPLC), Gas-Chromatography analysis, InfraRed spectroscopy analysis, Atomic Adsorption spectroscopy, UV-Vis spectroscopy. Analysis of chemical and physical parameters, such as tensile strength and corrosion, roughness analysis of raw plastic materials and silicone, titration and colorimetric analysis.
Bioinformatics/Informatics: Software for analysis of nucleic acids and proteins, screening of database of interest in molecular biology, primer designing, analysis of microRNA/gene targets. Daily use of web sites for scientific research.
Hazard Analysis & Critical Control Points (HACCP): Analysis and control of biological, chemical, and physical hazards from raw material to finished product, for food production.
1. “Epstein-Barr Virus MicroRNA Expression Increases Aggressiveness of Solid Malignancies” Pandya D, Mariani M, He S, Andreoli M, Spennato M, Dowell-Martino C, Fiedler P, Ferlini C. PLoS One. 2015 Sep 16.
2. “Class III β-tubulin in normal and cancer tissues” M Mariani, R Karki, M Spennato, D Pandya, S He, M Andreoli , P Fiedler ,C Ferlini . Gene. 2015 Jun 1;563(2):109-114.
3. “From protein communication to drug discovery” Persico M, Dato AD, Orteca N, Fattorusso C, Novellino E, Andreoli M, Ferlini C. Curr Top Med Chem. 2015 May 18.
4. “Identification of the first inhibitor of the GBP1:PIM1 interaction. Implications for the development of a new class of anticancer agents against paclitaxel resistant cancer cells”. Andreoli M, Persico M, Kumar A, Orteca N, Kumar V, Pepe A, Mahalingam S, Alegria AE, Petrella L, Sevciunaite L, Camperchioli A, Mariani M, Di Dato A, Novellino E, Scambia G, Malhotra SV, Ferlini C, Fattorusso C. Journal of medicinal chemestry, 2014.
5. “GTP is an allosteric modulator of the interaction between the guanylate-binding protein 1 and the prosurvival kinase PIM1” Marco Persico, Lella Petrella, Nausicaa Orteca, Antonio Di Dato, Marisa Mariani, Mirko Andreoli, Marta De Donato, Giovanni Scambia, Ettore Novellino, Cristiano Ferlini, Caterina Fattorusso . European Journal of Medicinal Chemistry. 25 July 2014.
6. “HGF/c-Met axis drives cancer aggressiveness in the neo-adjuvant setting of ovarian cancer” Marisa Mariani, Mark McHugh, Marco Petrillo, Steven Sieber, Shiquan He, Mirko Andreoli, Zheyang Wu, Paul Fiedler, Giovanni Scambia, Shohreh Shahabi, Cristiano Ferlini. Oncotarget Oncotarget. 2014 Jun 1st.
7. “Herpes Virus MicroRNA Expression and Significance in Serous Ovarian Cancer” D Pandya, M Mariani, M McHugh, M Andreoli, S Sieber, S He, C Dowell-Martino, P Fiedler, G Scambia, C Ferlini. PloS one, 2014.
8. “Integrated Multidimensional analysis is required for accurate prognostic biomarkers in colorectal cancer” Marisa Mariani, Shiquan He, Mark McHugh, Mirko Andreoli, Deep Pandya, Steven Sieber, Zheyang Wu, Paul Fiedler, Shohreh Shahabi, Cristiano Ferlini. PloS ONE PLoS One. 2014 July 2nd.
9. “βIII-Tubulin: biomarker of taxane resistance or drug target?” Karki R, Mariani M, Andreoli M, He S, Scambia G, Shahabi S, Ferlini C. Expert Opin Ther Targets. 2013 Apr;17(4):461-72.
10. “Novel drugs targeting microtubules: the role of epothilones” Ferrandina G, Mariani M, Andreoli M, Shahabi S, Scambia G, Ferlini C. Curr Pharm Des. 2012;18(19):2793-803.
11. “Adrenomedullin in Ovarian Cancer: Foe in vitro and Friend in vivo?" Cinzia Baranello; Marisa Mariani; Mirko Andreoli; Mara Fanelli; Enrica Martinelli; Gabriella Ferrandina; Giovanni Scambia; Shohreh Shahabi; Cristiano Ferlini. PloS ONE. PLoS One. 2012;7(7).
Molecular mechanisms of resistance to Patupilone in ovarian cancer. Presented at 31th NEAGO meeting, Stowe, VT, on June 11th , 2011. Andreoli M, Mariani M, Ferlini C, Shahabi.
Class III and class V β-tubulin are key elements of the cytoskeletal program of cell survival. Presented at 31th NEAGO meeting, Stowe, VT, on June 11th, 2011. Mariani M, Andreoli M, Shahabi S, Ferlini C.
A novel SPR method to develop GBP1:PIM1 inhibitors. Presented at 32nd Annual Meeting of the New England Association of Gynecologic Oncologists (NEAGO), Bar Harbor, Maine, on June 9th, 2012. Mirko Andreoli, Marisa Mariani, Marta De Donato, Caterina Fattorusso, Shohreh Shahabi, Cristiano Ferlini.
MiR-200c can mediate either poor or good outcome in ovarian cancer depending on HuR pattern of staining. Presented at 32nd Annual Meeting of the New England Association of Gynecologic Oncologists (NEAGO), Bar Harbor, Maine, on June 9th, 2012. Mariani M, Andreoli M, Shahabi S, Ferlini C.
HGF/c-Met axis drives cancer aggressiveness in the neo-adjuvant setting of ovarian cancer. Presented at AACR Annual Meeting 2014, April 5th-April 9th, San Diego, CA. Marisa Mariani, Mark McHugh, Marco Petrillo, Steven Sieber, Shiquan He, Mirko Andreoli, Paul Fiedler, Giovanni Scambia, Shohreh Shahabi, Cristiano Ferlini.
A multidimensional analysis of predictive biomarkers in colorectal cancer. Presented at AACR Annual Meeting 2014, April 5th-April 9th, San Diego, CA. Marisa Mariani, Shiquan He, Mark McHugh, Mirko Andreoli, Deep Pandya, Steven Sieber, Paul Fiedler, Shohreh Shahabi, Cristiano Ferlini.
Herpes virus microRNA expression and significance in serous ovarian cancer. Presented at AACR Annual Meeting 2015, April 18th-April 22th, Philadelphia, PA. Deep Pandya, Marisa Mariani, Mark McHugh, Mirko Andreoli, Steven Sieber, Shiquan He, Candice Dowell-Martino, Paul Fiedler, Giovanni Scambia, Cristiano Ferlini.
Italian: mother tongue. English: fluent speaking, reading and writing
Citizenship: Italian, US Permanent Resident (Permanent resident card).
research associate, scientist, molecular biology, molecular pathology, protein purification,